Leica SP5 X Laser Scanning Confocal Microscope

Leica SP5 X Laser Scanning Confocal Microscope

Leica SP5X

Leica SP5X

 

The SP5X uses a Koheras white light laser source in addition to regular short wavelength lasers
The Leica SP5X confocal system is equipped with multiple laser sources, including a 405 diode, an Argon laser (458, 476, 488, 496, 514nm) and a white light laser. The white light laser emits a broad spectrum of wavelengths from 470 to 670nm in 1 nm increments for simultaneous optimal excitation of multiple fluorophores.

Tunable excitation and tunable detection
Allow the user to image samples that have been labeled with any fluorophore. Spectral scanning allows optimization of excitation/emission wavelengths for new fluorophore, for combinations of different fluorophores, or to effectively avoid autofluorescence artifacts.

Tunable Prism-Based Spectral Detector and GaAsP hybrid detector
High speed resonant scanner and high sensitivity hybrid detector. The resonant scanner combined with Leica Hybrid (HyD) detector allows fast confocal imaging with minimum laser power to minimize photo-toxicity and bleaching.

High precision x-y and Z-Galvo motorized scanning stage
The microscope has a motorized stage that is capable of tile imaging for the entire section. Its high precision z-galvo Z stage, which has a minimum step size of 3nm and a travel range of 1500 µm, facilitates fast z-stack imaging for high-resolution 3D reconstruction.

Live-cell imaging with environmental chamber
The microscope has a transparent environmental chamber that enables users to prolong studies of living cells, including time-lapse image capture experiments for cell motility and cell death. Warm, filtered air circulates within the acrylic chamber from a heater unit that is mechanically isolated from the environmental chamber. Two top doors allow specimens to be changed. Another two doors below the stage allow access to the microscope setting controls.

Objectives

  • HC PL APO 10x/0.40 CS oo/0.17 /A
  • HC PL APO 20x/0.70 IMM CS
  • HC PL APO 40x/1.25-0.75 oil CS
  • HCX PL APO 63x/1.4-0.6 oil CS oo/0.17 /A
  • HCX PL APO 63x/1.2 water CS oo/0.14-0.18 /D
  • Detailed specs

MORE INFORMATION

For more information about the capabilities and use of this machine, please send an e-mail.

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